31 July 2019

Changes from v0.39.2

whitelist

bustools whitelist generates a whitelist based on the barcodes in a sorted BUS file.

project

The kallisto bus command maps reads to a set of transcripts. bustools project takes as input a sorted BUS output and a transcript to gene map (tr2g file), and outputs a BUS file, a matrix.ec file, and a list of genes, which collectively map each read to a set of genes.

linker

bustools linker removes specified section of barcode in BUS files.

inspect

A report summarizing the contents of a sorted BUS file can be output either to standard out or to a JSON file for further analysis using bustools inspect.

capture

The capture command has been updated to capture not only on a list of transcripts, but also a list of barcodes or UMIs.

Some minor bugfixes

  • Fixes issue #8 by not creating output directory
  • Mismatches in error reporting